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KMID : 0616120220530040226
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Korean Journal of Pharmacognosy
2022 Volume.53 No. 4 p.226 ~ p.233
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Development and Utilization of KASP Markers for the Identification of Three Types of Ephedra Herbs
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Baek Sun-Young
Lee Sun-Hee
Han Kyoung-Moon
Hwang Jin-Woo
Kim Hyung-Il
Baek Sun-Young
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Abstract
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Ephedra herbs are defined as stem of Ephedra sinica , Ephedra intermedia and Ephedra equisetina in the Korean Pharmacopoeia. It is important to use pure herbs to derive the safety and efficacy of herbal medicine. However, the identification of these herbs by conventional taxonomic methods is difficult. Recently, many studies have applied these DNA barcoding for the identification of herbal medicinal species using standard DNA markers. In this study, we report a case study in which the identification of Ephedra species was done by DNA barcoding. For identification of Ephedra species, 17 samples were collected, and a reference DNA barcode library was developed using 6 markers (rbcL, matK, ITS2, ycf1, ycf3, and rpoC2). To develop KASP-SNP markers, we selected 4 markers (ycf1, ycf3, rpl2, and rbcL), which were able to distinguish three Ephedra species. In the result, the specific markers for each of the three Ephedra were clustered into FAM-positive section, whereas non-targeted plants were clustered either HEX-positive or negative section. Therefore, we have developed KASP assay that allow rapid and easy Ephedra species identification using three KASP markers.
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KEYWORD
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Ephedra herb, DNA barcode, Herbal medicinal materials, Molecular identification, KASP assay
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